Dr. Jitendra Vishwakarma

Research Associate: Effects of maternal chronic intermittent hypoxia on the cholinergic system and cognitive function of offspring in rats in Electron Microscopy, Department of Anatomy, All India Institute of Medical Sciences (AIIMS) Delhi, India. PhD in Science with specialization in Neuroscience, Biotechnology and Toxicology from CSIR-Indian Institute of Toxicology Research, Lucknow, India (Registration from Academy of Scientific and Innovative Research (AcSIR) India October, 2023 continue September, 2023 Master’s in Science (Biotechnology) University of Calicut, Kerala, India July, 2015 Bachelor’s in Science (Zoology, Chemistry) Veer Bahadur Singh Purvanchal University, Jaunpur, India July, 2011 Animal handling: Maintenance of rodents, administration of chemicals through gavage, subcutaneous, intravenous, intraperitoneal injection.  Animal Surgeries: Stereotaxic injection in brain (Intra-cerebroventricular and intrahippocampaus and cerebellum), transcardial perfusion, dissection and isolation of brain.  Neurobehavioral tests: Learning and memory performances through Y-Maze Passive avoidance test, Grip Strength and Locomotor activity.  Cell Culture: Proficiency in mammalian primary cell cultures (Primary cultures of granule neurons, Hippocampal neurons, Astrocytes and Neuronal Stem Cells); Maintenance of mammalian cell lines (Neuro2A and PC12), siRNA and plasmid transfection.  Autophagy cell biology assays like Mito-GFP-RFP assay, mitotracker and lysotracker labeling and autophagy flux assay.  Molecular Techniques: Isolation of nucleic acids (DNA/RNA), cDNA preparation and real- time expression analysis, Western blotting, Immunofluorescence (Immunocytochemistry of primary neuron, astrocytes, neuron stem cells and neuronal cell line and Immunohistochemistry of brain sections).  Microscopy: Light, Confocal microscopy, fluorescent microscopy and Electron Microscopy